Monitoring COVID-19 Transmission Risks by Quantitative Real-Time PCR Tracing of Droplets in Hospital and Living Environments

Piana A, Colucci ME, Valeriani F, Marcolongo A, Sotgiu G, Pasquarella C, Margarucci LM, Petrucca A, Gianfranceschi G, Babudieri S, Vitali P, D’Ermo G, Bizzarro A, De Maio F, Vitali M, Azara A, Romano F, Simmaco M, Romano Spica V.
mSphere. 2021 Jan 6;6(1):e01070-20. doi: 10.1128/mSphere.01070-20. PMID: 33408231
Anthropic contamination by droplets and biological fluids was monitored by detecting the microbiota signature using multiplex quantitative real-time PCR (qPCR) on selected species and massive sequencing on 16S amplicons. …We monitored by qPCR or next generation sequencin …